Why Is ProHydrolase Important?

Adding proteolytic enzymes like ProHydrolase to protein powder improves digestion, increases blood amino acid levels and decreases digestive discomfort. A clinical study showed that consuming 26 grams of whey protein along with 250 mg of ProHydrolase increased amino acid levels in the blood 20% more than whey protein alone.

The ProHydrolase blend consists of Bacillus subtilis, papain and serratiopeptidase that are well-studied and generally considered safe. This patented proteolytic enzyme mixture is effective with whey, casein, soy, egg and hemp proteins.

Increases Amino Acids in the Blood

Protein is a complex macronutrient that helps build and repair muscle and tissue. However, it is only effective when it is absorbed by the body. A specialized enzyme blend called Prohydrolase has been shown to increase amino acid uptake from a protein supplement and minimize digestive discomfort.

Studies show that Prohydrolase enhances the digestion and absorption of whey, casein, egg, soy, hemp and pea proteins. It breaks down protein into smaller peptides and free-form amino acids that are readily available to the body for use.

The Prohydrolase enzyme blend contains the bacteria Bacillus subtilis and Ananas comosus/bromelain. Both of these are well-studied and generally recognized as safe ingredients for use in food products. Moreover, Ananas comosus/bromelain is approved by the European Medicines Agency for use in NexoBrid, a treatment for severe skin burns. Furthermore, Bacillus subtilis is a well-studied and widely used bacterium as a probiotic.

Reduces C-Reactive Protein

Prohydrolase helps to break down protein into smaller peptides and amino acids. These are then absorbed by the digestive tract. This allows the protein to be digested more quickly. It also reduces stomach discomfort. This enzyme also increases the amount of leucine available to the body, which is important for muscle growth and recovery.

Many brands of protein powders advertise that they use this ingredient. However, they rarely disclose how much is used. CytoSport Monster Blend, for example, uses 125mg of Prohydrolase per 25g of milk protein. Other brands, such as Activ Folks, use a lower concentration of this enzyme and do not disclose it on their label.

Numerous in vitro studies and two separate clinical trials have shown that the ability of this protein enzyme to rapidly break down proteins into bio-usable forms will significantly increase amino acid availability, decrease C-reactive protein levels and decrease large peptides responsible for digestive discomfort associated with consuming protein. The enzyme works well with whey, casein, soy, egg, pea and hemp proteins.

Reduces Large Peptides

The proteases in Prohydrolase are able to break down proteins into small peptides which can then be easily digested by the digestive system. This allows for the protein to be better absorbed into the body, and it also makes the amino acids more readily available for muscle growth, repair and recovery.

The enzymes in Prohydrolase can even help break down large protein particles which can cause stomach discomfort after consuming protein such as gas, bloating and abdominal pain. It can also increase the availability of the essential amino acid leucine, which is known to boost protein synthesis and speed up the rate at which muscles build and recover.

A clinical study has shown that consuming whey protein with Prohydrolase in it significantly increases the amount of amino acids in the blood. Prohydrolase is a protease blend that has been created by Deerland Enzymes and contains Bacillus subtilis and Bromelain which are both well-studied, safe ingredients for use as supplements.

Increases Leucine Levels

Branched-chain amino acid (BCAA) leucine plays a major role in protein synthesis, glucose tolerance and fat reduction. Optimal levels of leucine increase muscle protein accretion and reduce appetite. Prohydrolase increases leucine availability by breaking down proteins into smaller peptides for better digestion.

One clinical study by Deerland Probiotics & Enzymes found that adding a blend of their enzymes, including Prohydrolase, to whey protein increased amino acids in the blood by 20 percent compared to consuming whey protein alone. It also decreased C-reactive protein levels that indicate inflammation and didn’t affect insulin levels.


The Prohydrolase blend is a mix of protease enzymes that include Bacillus subtilis and Ananas comosus/Bromelain, both of which are well-studied and considered safe for consumption. This enzyme blend is widely used in the food industry and as a meat tenderizer. Many dietary supplement companies advertise that they use the enzyme blend but don’t disclose how much of it is in their products, so customers don’t know if they are getting its benefits.